验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, PC-3 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | SW480 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20437-1-AP targets RNF6 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | RNF6 fusion protein Ag14209 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein (C3H2C3 type) 6 |
| 别名 | SPG2, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF6 |
| 计算分子量 | 685 aa, 78 kDa |
| 观测分子量 | 78 kDa |
| GenBank蛋白编号 | BC034688 |
| 基因名称 | RNF6 |
| Gene ID (NCBI) | 6049 |
| RRID | AB_10666853 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y252 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF6 was firstly cloned in a genetic study of chromosomal rearrangements in myeloproliferative disorders, mapping to chromosome 13q12.12 [PMID:10331950]. It containing a coiled-coil domain at the N-terminus, and a RING-H2 finger at the C-terminus which is responsible for its ubiquitin ligase activity. It also activate Inha gene expression by participating in a protein complex binding to the promoter region [PMID:11971979]. In addition, LIMK1 is a substrate of RNF6 and the RNF6-induced LIMK1 polyubiquitination is mediated via K48 of ubiquitin and leads to proteasomal degradation of the kinase [PMID:16204183].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF6 antibody 20437-1-AP | Download protocol |
| IF protocol for RNF6 antibody 20437-1-AP | Download protocol |
| IP protocol for RNF6 antibody 20437-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene RING-finger protein 6 promotes colorectal tumorigenesis by transcriptionally activating SF3B2.
| ||
Cancer Lett Ring finger protein 6 enhances chemo-resistance by transcriptionally activating proliferating cell nuclear antigen expression and attenuating DNA damage in lung adenocarcinoma. | ||
Acta Pharmacol Sin RNF6 promotes myeloma cell proliferation and survival by inducing glucocorticoid receptor polyubiquitination. | ||
Anticancer Drugs miR-203a suppresses cell proliferation by targeting RING-finger protein 6 in colorectal cancer. | ||
J Biol Chem Induction of zinc finger protein RNF6 auto-ubiquitination for the treatment of myeloma and chronic myeloid leukemia | ||
Int J Biol Sci TRIM26 promotes non-small cell lung cancer survival by inducing PBX1 degradation |
