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  • Featured Product
  • KD/KO Validated

RNH1 Polyclonal antibody

RNH1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10345-1-AP

Print datasheet

Synonyms

RNH, Ribonuclease/angiogenin inhibitor 1, Ribonuclease inhibitor, RAI, PRI

验证数据展示

  • WB analysis using 10345-1-AP

    WB analysis using 10345-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa cells using 10345-1-AP

    WB analysis of HeLa cells using 10345-1-AP

    WB result of RNH1 antibody (10345-1-AP, 1:2500) with si-control and si-RNH1 transfected HeLa cells.

  • IHC staining of mouse liver using 10345-1-AP

    IHC staining of mouse liver using 10345-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10345-1-AP (RNH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 10345-1-AP

    IHC staining of mouse liver using 10345-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10345-1-AP (RNH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 10345-1-AP

    IF Staining of MCF-7 using 10345-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RNH1 antibody (10345-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse liver using 10345-1-AP

    IP experiment of mouse liver using 10345-1-AP

    IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:500) with mouse liver tissue lysate 6400ug.

  • IP experiment of HEK-293T using 10345-1-AP

    IP experiment of HEK-293T using 10345-1-AP

    IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with HEK-293T cells lysate 2040 ug.

  • IP experiment of mouse liver using 10345-1-AP

    IP experiment of mouse liver using 10345-1-AP

    IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with mouse liver tissue lysate 1840 ug.

  • IP experiment of rat liver using 10345-1-AP

    IP experiment of rat liver using 10345-1-AP

    IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with rat liver tissue lysate 5520 ug.

  • FC experiment of MCF-7 using 10345-1-AP

    FC experiment of MCF-7 using 10345-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human RNH1 (10345-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, human brain tissue, human heart tissue, human liver tissue, L02 cells, HepG2 cells, Jurkat cells, human placenta tissue
Positive IP detected inmouse liver tissue, HEK-293T cells, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10345-1-AP targets RNH1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RNH1 fusion protein Ag0350 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribonuclease/angiogenin inhibitor 1
Synonyms RNH, Ribonuclease/angiogenin inhibitor 1, Ribonuclease inhibitor, RAI, PRI
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC000677
Gene Symbol RNH1
Gene ID (NCBI) 6050
RRIDAB_2269733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13489
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribonuclease/ angiogenin inhibitor (RNH, synonyms: RAI, MGC4569, MGC18200, MGC54054) is a protein that binds tightly to ribonucleases in cells and may be essential in the control of mRNA degradation and gene expression. The human RNH gene has been regionally localized to chromosome band 11p15 by in situ hybridization.

实验方案

Product Specific Protocols
WB protocol for RNH1 antibody 10345-1-APDownload protocol
IHC protocol for RNH1 antibody 10345-1-APDownload protocol
IF protocol for RNH1 antibody 10345-1-APDownload protocol
IP protocol for RNH1 antibody 10345-1-APDownload protocol
FC protocol for RNH1 antibody 10345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

EMBO J

Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.

Authors - Rongpan Bai
View Article
humanWB

J Cell Biol

Angiogenin cleaves tRNA and promotes stress-induced translational repression.

Authors - Yamasaki Satoshi S
  • KD Validated
View Article
humanWB

Proc Natl Acad Sci U S A

Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers.

Authors - Shozo Honda
View Article
humanWB

Int J Mol Sci

RTCB Complex Regulates Stress-Induced tRNA Cleavage

Authors - Yasutoshi Akiyama
View Article
humanIHC

J Proteome Res

Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE.

Authors - Stephens Andrew N AN
View Article
mouseWB

J Biol Chem

Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress.

Authors - Saikia Mridusmita M
View Article