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验证数据展示

  • WB analysis of 3T3-L1 using 25820-1-AP

    WB analysis of 3T3-L1 using 25820-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 25820-1-AP (RNPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse spleen using 25820-1-AP

    IP experiment of mouse spleen using 25820-1-AP

    IP result of anti-RNPC3 (IP:25820-1-AP, 4ug; Detection:25820-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

  • Featured Product
  • KD/KO验证

RNPC3 Polyclonal antibody

RNPC3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25820-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

KIAA183, KIAA1839, RBM40, RNA binding motif protein 40, RNA binding protein 40, RNP, RNPC3, U11/U12 65K, U11/U12 snRNP 65 kDa protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in3T3-L1 cells, mouse spleen tissue
Positive IP detected inmouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25820-1-AP targets RNPC3 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 RNPC3 fusion protein Ag22957 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA-binding region (RNP1, RRM) containing 3
别名 KIAA183, KIAA1839, RBM40, RNA binding motif protein 40, RNA binding protein 40, RNP, RNPC3, U11/U12 65K, U11/U12 snRNP 65 kDa protein
计算分子量 517 aa, 59 kDa
观测分子量 62-65 kDa
GenBank蛋白编号BC010697
基因名称 RNPC3
Gene ID (NCBI) 55599
RRIDAB_2880254
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96LT9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNPC3 antibody 25820-1-APDownload protocol
IP protocol for RNPC3 antibody 25820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Genet

Alternative exon definition events control the choice between nuclear retention and cytoplasmic export of U11/U12-65K mRNA.

Authors - Jens Verbeeren
View Article
mouseWB

RNA

Early developmental arrest and impaired gastrointestinal homeostasis in U12-dependent splicing-defective Rnpc3-deficient mice.

Authors - Karen Doggett
  • KO Validated
View Article