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  • Featured Product
  • KD/KO Validated

RNPC3 Polyclonal antibody

RNPC3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25820-1-AP

Print datasheet

Synonyms

KIAA183, KIAA1839, RBM40, RNA binding motif protein 40, RNA binding protein 40, RNP, RNPC3, U11/U12 65K, U11/U12 snRNP 65 kDa protein

验证数据展示

  • WB analysis of 3T3-L1 using 25820-1-AP

    WB analysis of 3T3-L1 using 25820-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 25820-1-AP (RNPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse spleen using 25820-1-AP

    IP experiment of mouse spleen using 25820-1-AP

    IP result of anti-RNPC3 (IP:25820-1-AP, 4ug; Detection:25820-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

经过测试的应用

Positive WB detected in3T3-L1 cells, mouse spleen tissue
Positive IP detected inmouse spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25820-1-AP targets RNPC3 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen RNPC3 fusion protein Ag22957 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA-binding region (RNP1, RRM) containing 3
Synonyms KIAA183, KIAA1839, RBM40, RNA binding motif protein 40, RNA binding protein 40, RNP, RNPC3, U11/U12 65K, U11/U12 snRNP 65 kDa protein
Calculated Molecular Weight 517 aa, 59 kDa
Observed Molecular Weight 62-65 kDa
GenBank Accession NumberBC010697
Gene Symbol RNPC3
Gene ID (NCBI) 55599
RRIDAB_2880254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LT9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNPC3 antibody 25820-1-APDownload protocol
IP protocol for RNPC3 antibody 25820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Genet

Alternative exon definition events control the choice between nuclear retention and cytoplasmic export of U11/U12-65K mRNA.

Authors - Jens Verbeeren
View Article
mouseWB

RNA

Early developmental arrest and impaired gastrointestinal homeostasis in U12-dependent splicing-defective Rnpc3-deficient mice.

Authors - Karen Doggett
  • KO Validated
View Article