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验证数据展示

  • WB analysis using 14764-1-AP

    WB analysis using 14764-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14764-1-AP (RNPEP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 14764-1-AP

    IP experiment of A549 using 14764-1-AP

    IP result of anti-RNPEP (IP:14764-1-AP, 4ug; Detection:14764-1-AP 1:500) with A549 cells lysate 2000ug.

  • IHC staining of human lung cancer using 14764-1-AP

    IHC staining of human lung cancer using 14764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14764-1-AP (RNPEP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 14764-1-AP

    IHC staining of human lung cancer using 14764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14764-1-AP (RNPEP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

RNPEP Polyclonal antibody

RNPEP Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14764-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

EC:3.4.11.6, AP-B, APB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, HepG2 cells, A549 cells, HeLa cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14764-1-AP targets RNPEP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 RNPEP fusion protein Ag6526 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 arginyl aminopeptidase (aminopeptidase B)
别名 EC:3.4.11.6, AP-B, APB
计算分子量 72 kDa
观测分子量 72 kDa
GenBank蛋白编号BC001064
基因名称 RNPEP
Gene ID (NCBI) 6051
RRIDAB_2254005
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H4A4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNPEP antibody 14764-1-APDownload protocol
IHC protocol for RNPEP antibody 14764-1-APDownload protocol
IP protocol for RNPEP antibody 14764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Autism

RNA sequencing and proteomics approaches reveal novel deficits in the cortex of Mecp2-deficient mice, a model for Rett syndrome.

Authors - Natasha L Pacheco
View Article
humanWB

Cancer Sci

Tumor exosomal RNPEP promotes lung metastasis of liver cancer via inducing cancer-associated fibroblast activation

Authors - Yuankun Chen
  • KD Validated
View Article