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ROBO1 Polyclonal antibody

ROBO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25181-1-AP

Print datasheet

Synonyms

Deleted in U twenty twenty, DUTT1, H Robo 1, ROBO1, Roundabout homolog 1, SAX3

验证数据展示

  • WB analysis of Y79 using 25181-1-AP

    WB analysis of Y79 using 25181-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 25181-1-AP (ROBO1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 25181-1-AP

    IHC staining of human brain using 25181-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25181-1-AP (ROBO1 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 25181-1-AP

    IHC staining of human brain using 25181-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25181-1-AP (ROBO1 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inY79 cells, HeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25181-1-AP targets ROBO1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen ROBO1 fusion protein Ag16461 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name roundabout, axon guidance receptor, homolog 1 (Drosophila)
Synonyms Deleted in U twenty twenty, DUTT1, H Robo 1, ROBO1, Roundabout homolog 1, SAX3
Calculated Molecular Weight 1651 aa, 181 kDa
Observed Molecular Weight 200-250 kDa
GenBank Accession NumberBC112336
Gene Symbol ROBO1
Gene ID (NCBI) 6091
RRIDAB_2879946
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6N7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ROBO1, also named as DUTT1, belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. It may be required for lung development.

实验方案

Product Specific Protocols
WB protocol for ROBO1 antibody 25181-1-APDownload protocol
IHC protocol for ROBO1 antibody 25181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols