• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 25181-1-AP

    WB analysis using 25181-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25181-1-AP (ROBO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25181-1-AP

    IHC staining of mouse brain using 25181-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25181-1-AP (ROBO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 25181-1-AP

    IF Staining of mouse brain using 25181-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ROBO1 antibody (25181-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 25181-1-AP

    IHC staining of human brain using 25181-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25181-1-AP (ROBO1 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 25181-1-AP

    IHC staining of human brain using 25181-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25181-1-AP (ROBO1 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 25181-1-AP

    IF Staining of HeLa using 25181-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ROBO1 antibody (25181-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

ROBO1 Polyclonal antibody

ROBO1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 25181-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

SAX3, Roundabout homolog 1, H-Robo-1, H Robo 1, DUTT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Y79 cells
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25181-1-AP targets ROBO1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 ROBO1 fusion protein Ag16461 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 roundabout, axon guidance receptor, homolog 1 (Drosophila)
别名 SAX3, Roundabout homolog 1, H-Robo-1, H Robo 1, DUTT1
计算分子量 1651 aa, 181 kDa
观测分子量200-250 kDa
GenBank蛋白编号BC112336
基因名称 ROBO1
Gene ID (NCBI) 6091
RRIDAB_2879946
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6N7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ROBO1, also named as DUTT1, belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. It may be required for lung development.

实验方案

Product Specific Protocols
WB protocol for ROBO1 antibody 25181-1-APDownload protocol
IHC protocol for ROBO1 antibody 25181-1-APDownload protocol
IF protocol for ROBO1 antibody 25181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols