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验证数据展示

  • WB analysis using 25181-1-AP

    WB analysis using 25181-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25181-1-AP (ROBO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25181-1-AP

    IHC staining of mouse brain using 25181-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25181-1-AP (ROBO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 25181-1-AP

    IF Staining of mouse brain using 25181-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ROBO1 antibody (25181-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 25181-1-AP

    IHC staining of human brain using 25181-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25181-1-AP (ROBO1 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 25181-1-AP

    IHC staining of human brain using 25181-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25181-1-AP (ROBO1 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 25181-1-AP

    IF Staining of HeLa using 25181-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ROBO1 antibody (25181-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

ROBO1 Polyclonal antibody

ROBO1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 25181-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

SAX3, Roundabout homolog 1, H-Robo-1, H Robo 1, DUTT1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Y79 cells
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25181-1-AP targets ROBO1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ROBO1 fusion protein Ag16461 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name roundabout, axon guidance receptor, homolog 1 (Drosophila)
Synonyms SAX3, Roundabout homolog 1, H-Robo-1, H Robo 1, DUTT1
Calculated Molecular Weight 1651 aa, 181 kDa
Observed Molecular Weight200-250 kDa
GenBank Accession NumberBC112336
Gene Symbol ROBO1
Gene ID (NCBI) 6091
RRIDAB_2879946
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6N7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ROBO1, also named as DUTT1, belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. It may be required for lung development.

实验方案

Product Specific Protocols
WB protocol for ROBO1 antibody 25181-1-APDownload protocol
IHC protocol for ROBO1 antibody 25181-1-APDownload protocol
IF protocol for ROBO1 antibody 25181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols