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ROBO1-Specific Polyclonal antibody
ROBO1-Specific Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20219-1-AP targets ROBO1-Specific in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | roundabout, axon guidance receptor, homolog 1 (Drosophila) |
| Synonyms | Deleted in U twenty twenty, DUTT1, H Robo 1, ROBO1, ROBO1-Specific, Roundabout homolog 1, SAX3 |
| Calculated Molecular Weight | 181 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | NM_002941 |
| Gene Symbol | ROBO1 |
| Gene ID (NCBI) | 6091 |
| RRID | AB_10694675 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6N7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ROBO1, also named as DUTT1, belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. It may be required for lung development. The antibody is specific to ROBO1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ROBO1-Specific antibody 20219-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins. | ||
Mol Cell AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.
| ||
Chin Med J (Engl) Repression of Smad3 by silencing Robo1 attenuates epithelial-mesenchymal transition in tracheobronchial stenosis | ||
Aging (Albany NY) MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma.
| ||
Angiogenesis Slit2/Robo1 signaling is involved in angiogenesis of glomerular endothelial cells exposed to a diabetic-like environment. | ||
iScience Activation of EPHA2-ROBO1 Heterodimer by SLIT2 Attenuates Non-canonical Signaling and Proliferation in Squamous Cell Carcinomas.
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