ROBO4-Specific Polyclonal antibody
ROBO4-Specific Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
20221-1-AP targets ROBO4-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | roundabout homolog 4, magic roundabout (Drosophila) |
| Synonyms | ECSM4, Magic roundabout, MRB, ROBO4, ROBO4-Specific, Roundabout homolog 4 |
| Calculated Molecular Weight | 107 kDa |
| Observed Molecular Weight | 90-170 kDa |
| GenBank Accession Number | NM_019055 |
| Gene Symbol | ROBO4 |
| Gene ID (NCBI) | 54538 |
| RRID | AB_10665944 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WZ75 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ROBO4 belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. ROBO4 may mediate the inhibition of primary endothelial cell migration by Slit proteins. This antibody is specific to ROBO4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ROBO4-Specific antibody 20221-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Biol Lett Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions | ||
J Transl Med The role of TET2-mediated ROBO4 hypomethylation in the development of diabetic retinopathy | ||
Mol Ther Nucleic Acids Role of ANKHD1/LINC00346/ZNF655 Feedback Loop in Regulating the Glioma Angiogenesis via Staufen1-Mediated mRNA Decay. |
