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ROCK1 Monoclonal antibody, PBS Only

ROCK1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G5E12

Cat no : 66782-1-PBS

Print datasheet

Synonyms

p160 ROCK 1, P160ROCK, PRO0435, ROCK1

验证数据展示

  • WB analysis using 66782-1-Ig (same clone as 66782-1-PBS)

    WB analysis using 66782-1-Ig (same clone as 66782-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66782-1-Ig (ROCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66782-1-PBS in a different storage buffer formulation.

产品信息

66782-1-PBS targets ROCK1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ROCK1 fusion protein Ag16317 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Rho-associated, coiled-coil containing protein kinase 1
Synonyms p160 ROCK 1, P160ROCK, PRO0435, ROCK1
Calculated Molecular Weight 1354 aa, 158 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC113114
Gene Symbol ROCK1
Gene ID (NCBI) 6093
RRIDAB_2882127
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13464
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.