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ROCK1 Recombinant antibody, PBS Only (Capture)

ROCK1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230423H5

Cat no : 83166-4-PBS

Print datasheet

Synonyms

Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1, EC:2.7.11.1

验证数据展示

  • Sandwich ELISA standard curve of MP00229-3

    Sandwich ELISA standard curve of MP00229-3

    Sandwich ELISA standard curve of MP00229-3, Human ROCK1 Recombinant Matched Antibody Pair - PBS only. 83166-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16317. 83166-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00229-3

    Sample test of MP00229-3

    The mean ROCK1 concentration was determined to be 4,443.0 pg/mL in A431 cell extract based on a 2.6 mg/mL extract load and 13,265.0 pg/mL in HepG2 cell extract based on a 5.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP00229-1

    Cytometric bead array standard curve of MP00229-1

    Cytometric bead array standard curve of MP00229-1, ROCK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83166-4-PBS. Detection antibody: 83166-2-PBS. Standard: Ag16317. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83166-4-PBS

    Affinity and Kinetic Characterization of 83166-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 83166-4-PBS against Human ROCK1 was performed. The affinity constant is 1.56 nM.

产品信息

83166-4-PBS targets ROCK1 as part of a matched antibody pair:

MP00229-1: 83166-4-PBS capture and 83166-2-PBS detection (validated in Cytometric bead array)

MP00229-3: 83166-4-PBS capture and 83166-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen ROCK1 fusion protein Ag16317 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Rho-associated, coiled-coil containing protein kinase 1
Synonyms Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1, EC:2.7.11.1
Calculated Molecular Weight 1354 aa, 158 kDa
GenBank Accession NumberBC113114
Gene Symbol ROCK1
Gene ID (NCBI) 6093
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13464
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C