• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • Sandwich ELISA standard curve of MP00229-3

    Sandwich ELISA standard curve of MP00229-3

    Sandwich ELISA standard curve of MP00229-3, Human ROCK1 Recombinant Matched Antibody Pair - PBS only. 83166-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16317. 83166-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00229-3

    Sample test of MP00229-3

    The mean ROCK1 concentration was determined to be 4,443.0 pg/mL in A431 cell extract based on a 2.6 mg/mL extract load and 13,265.0 pg/mL in HepG2 cell extract based on a 5.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP00229-1

    Cytometric bead array standard curve of MP00229-1

    Cytometric bead array standard curve of MP00229-1, ROCK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83166-4-PBS. Detection antibody: 83166-2-PBS. Standard: Ag16317. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83166-4-PBS

    Affinity and Kinetic Characterization of 83166-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 83166-4-PBS against Human ROCK1 was performed. The affinity constant is 1.56 nM.

ROCK1 Recombinant antibody, PBS Only (Capture)

ROCK1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83166-4-PBS

产品说明书

CloneNo. 230423H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1, EC:2.7.11.1

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

83166-4-PBS targets ROCK1 as part of a matched antibody pair:

MP00229-1: 83166-4-PBS capture and 83166-2-PBS detection (validated in Cytometric bead array)

MP00229-3: 83166-4-PBS capture and 83166-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 ROCK1 fusion protein Ag16317 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Rho-associated, coiled-coil containing protein kinase 1
别名 Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1, EC:2.7.11.1
计算分子量 1354 aa, 158 kDa
GenBank蛋白编号BC113114
基因名称 ROCK1
Gene ID (NCBI) 6093
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13464
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C