Featured Product
KD/KO Validated

ROCK2(middle) Polyclonal antibody

ROCK2(middle) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat no : 21645-1-AP

Print datasheet

Synonyms

ROCK2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2

验证数据展示

WB analysis of HT-1080 using 21645-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21645-1-AP
WB result of ROCK2(middle) antibody (21645-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2(middle) transfected HeLa cells.
IP experiment of HeLa using 21645-1-AP
IP result of anti-ROCK2(middle) (IP:21645-1-AP, 5ug; Detection:21645-1-AP 1:700) with HeLa cells lysate 1320ug.
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21645-1-AP (ROCK2(middle) antibody at dilution of 1:200 (under 10x lens).
Ready-to-use IHC kits available with ROCK2 antibody included
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21645-1-AP (ROCK2(middle) antibody at dilution of 1:200 (under 40x lens).
Ready-to-use IHC kits available with ROCK2 antibody included
IF Staining of HepG2 using 21645-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 21645-1-AP

Size

点击咨询在线客服

经过测试的应用

Positive WB detected in	HT-1080 cells, mouse brain tissue, HeLa cells, mouse lung tissue, MCF-7 cells, mouse liver tissue, L02 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, Hela cells

Planning an IHC experiment? We recommend our IHCeasy ROCK2 Ready-To-Use IHC Kit. ROCK2 primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 21 publications below
IHC	See 4 publications below
IF	See 5 publications below
IP	See 2 publications below

产品信息

21645-1-AP targets ROCK2(middle) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	ROCK2(middle) fusion protein Ag16330 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	Rho-associated, coiled-coil containing protein kinase 2
Synonyms	ROCK2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2
Calculated Molecular Weight	77 kDa, 161 kDa
Observed Molecular Weight	161 kDa
GenBank Accession Number	BC111801
Gene Symbol	ROCK2
Gene ID (NCBI)	9475
RRID	AB_10858624
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75116
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).

实验方案

Product Specific Protocols
WB protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol
IHC protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol
IF protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol
IP protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IHC	Oncotarget Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis. Authors - Rachel Hiu Ha Ching View Article
rat	WB,IF	Neurochem Res Pre-electroacupuncture Ameliorates Cerebral Ischemia-reperfusion Injury by Inhibiting Microglial RhoA/pyrin/GSDMD Signaling Pathway Authors - Hao Fang View Article
human	WB	Cells The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells. Authors - Kathryn N Becker View Article
rat	WB	Front Pharmacol Liraglutide Ameliorates Erectile Dysfunction via Regulating Oxidative Stress, the RhoA/ROCK Pathway and Autophagy in Diabetes Mellitus. Authors - Penghui Yuan View Article
mouse	WB	Mol Pharmacol Megakaryocytic Smad4 Regulates Platelet Function Through Syk and ROCK2 Expression. Authors - Yanhua Wang View Article
rat	WB	J Sex Med FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis. Authors - Kai Cui View Article

产品中心

应用中心

研究领域

cGMP 级蛋白

技术支持

促销活动

关于我们

抗体

免疫分析

PTG-Lab免疫学实验试剂

PTG-Cell细胞实验试剂

细胞因子&活性蛋白

重组蛋白

纳米抗体相关产品

空间多组学

表观遗传领域相关产品

研究领域

ROCK2(middle) Polyclonal antibody

ROCK2(middle) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

验证数据展示

WB analysis of HT-1080 using 21645-1-AP

WB analysis of HeLa using 21645-1-AP

IP experiment of HeLa using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IF Staining of HepG2 using 21645-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis.

Pre-electroacupuncture Ameliorates Cerebral Ischemia-reperfusion Injury by Inhibiting Microglial RhoA/pyrin/GSDMD Signaling Pathway

The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.

Liraglutide Ameliorates Erectile Dysfunction via Regulating Oxidative Stress, the RhoA/ROCK Pathway and Autophagy in Diabetes Mellitus.

Megakaryocytic Smad4 Regulates Platelet Function Through Syk and ROCK2 Expression.

FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.