验证数据展示
经过测试的应用
| Positive WB detected in | HT-1080 cells, mouse brain tissue, HeLa cells, mouse lung tissue, MCF-7 cells, mouse liver tissue, L02 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, Hela cells |
Planning an IHC experiment? We recommend our IHCeasy ROCK2 Ready-To-Use IHC Kit. ROCK2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21645-1-AP targets ROCK2(middle) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ROCK2(middle) fusion protein Ag16330 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rho-associated, coiled-coil containing protein kinase 2 |
| 别名 | ROCK2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2 |
| 计算分子量 | 77 kDa, 161 kDa |
| 观测分子量 | 161 kDa |
| GenBank蛋白编号 | BC111801 |
| 基因名称 | ROCK2 |
| Gene ID (NCBI) | 9475 |
| RRID | AB_10858624 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75116 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ROCK2(middle) antibody 21645-1-AP | Download protocol |
| IHC protocol for ROCK2(middle) antibody 21645-1-AP | Download protocol |
| IF protocol for ROCK2(middle) antibody 21645-1-AP | Download protocol |
| IP protocol for ROCK2(middle) antibody 21645-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci TAGLN-RhoA/ROCK2-SLC2A3-mediated Mechano-metabolic Axis Promotes Skin Fibrosis | ||
Oncotarget Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis. | ||
Neurochem Res Pre-electroacupuncture Ameliorates Cerebral Ischemia-reperfusion Injury by Inhibiting Microglial RhoA/pyrin/GSDMD Signaling Pathway | ||
Cells The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells. | ||
Front Pharmacol Liraglutide Ameliorates Erectile Dysfunction via Regulating Oxidative Stress, the RhoA/ROCK Pathway and Autophagy in Diabetes Mellitus. | ||
Mol Pharmacol Megakaryocytic Smad4 Regulates Platelet Function Through Syk and ROCK2 Expression. |
