• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ROCK2 Monoclonal antibody, PBS Only

ROCK2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C7B8

Cat no : 66633-1-PBS

Print datasheet

Synonyms

KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2

验证数据展示

  • WB analysis using 66633-1-Ig (same clone as 66633-1-PBS)

    WB analysis using 66633-1-Ig (same clone as 66633-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66633-1-Ig (ROCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66633-1-Ig (same clone as 66633-1-PBS)

    WB analysis of HeLa using 66633-1-Ig (same clone as 66633-1-PBS)

    WB result of ROCK2 antibody (66633-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2 transfected HeLa cells. This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ROCK2 antibody included
  • IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ROCK2 antibody included
Planning an IHC experiment? We recommend our IHCeasy ROCK2 Ready-To-Use IHC Kit. ROCK2 primary antibody included.

产品信息

66633-1-PBS targets ROCK2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen ROCK2 fusion protein Ag16330 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Rho-associated, coiled-coil containing protein kinase 2
Synonyms KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2
Calculated Molecular Weight 77 kDa, 161 kDa
Observed Molecular Weight 161 kDa
GenBank Accession NumberBC111801
Gene Symbol ROCK2
Gene ID (NCBI) 9475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75116
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.