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验证数据展示

  • WB analysis using 66633-1-Ig (same clone as 66633-1-PBS)

    WB analysis using 66633-1-Ig (same clone as 66633-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66633-1-Ig (ROCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66633-1-Ig (same clone as 66633-1-PBS)

    WB analysis of HeLa using 66633-1-Ig (same clone as 66633-1-PBS)

    WB result of ROCK2 antibody (66633-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2 transfected HeLa cells. This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ROCK2 antibody included
  • IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ROCK2 antibody included
  • Featured Product
  • KD/KO验证

ROCK2 Monoclonal antibody, PBS Only

ROCK2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66633-1-PBS

产品说明书

CloneNo. 1C7B8

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Rat, Mouse

应用

WB, IHC, Indirect ELISA

别名

KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ROCK2 Ready-To-Use IHC Kit. ROCK2 primary antibody included.

产品信息

66633-1-PBS targets ROCK2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Rat, Mouse
免疫原 ROCK2 fusion protein Ag16330 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Rho-associated, coiled-coil containing protein kinase 2
别名 KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2
计算分子量 77 kDa, 161 kDa
观测分子量 161 kDa
GenBank蛋白编号BC111801
基因名称 ROCK2
Gene ID (NCBI) 9475
RRIDAB_2881992
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75116
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.