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验证数据展示

  • WB analysis of HeLa using 20248-1-AP (same clone as 20248-1-PBS)

    WB analysis of HeLa using 20248-1-AP (same clone as 20248-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

    IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ROCK2 antibody included
  • IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

    IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ROCK2 antibody included
  • IF Staining of HepG2 using 20248-1-AP (same clone as 20248-1-PBS)

    IF Staining of HepG2 using 20248-1-AP (same clone as 20248-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using ROCK2 antibody 20248-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ROCK2-Specific(C-term) Polyclonal antibody, PBS Only

ROCK2-Specific(C-term) Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20248-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ROCK2 Ready-To-Use IHC Kit. ROCK2 primary antibody included.

产品信息

20248-1-PBS targets ROCK2-Specific(C-term) in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho-associated, coiled-coil containing protein kinase 2
别名
计算分子量 161 kDa
观测分子量 164 kDa
GenBank蛋白编号NM_004850
基因名称 ROCK2
Gene ID (NCBI) 9475
RRIDAB_10665551
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75116
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.