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验证数据展示

  • WB analysis of HeLa using 20248-1-AP (same clone as 20248-1-PBS)

    WB analysis of HeLa using 20248-1-AP (same clone as 20248-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

    IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ROCK2 antibody included
  • IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

    IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ROCK2 antibody included
  • IF Staining of HepG2 using 20248-1-AP (same clone as 20248-1-PBS)

    IF Staining of HepG2 using 20248-1-AP (same clone as 20248-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using ROCK2 antibody 20248-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ROCK2-Specific(C-term) Polyclonal antibody, PBS Only

ROCK2-Specific(C-term) Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20248-1-PBS

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ROCK2 Ready-To-Use IHC Kit. ROCK2 primary antibody included.

产品信息

20248-1-PBS targets ROCK2-Specific(C-term) in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho-associated, coiled-coil containing protein kinase 2
Synonyms
Calculated Molecular Weight 161 kDa
Observed Molecular Weight 164 kDa
GenBank Accession NumberNM_004850
Gene Symbol ROCK2
Gene ID (NCBI) 9475
RRIDAB_10665551
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75116
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.