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ROD1 Polyclonal antibody
ROD1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, mouse thymus tissue, HeLa cells, Jurkat cells, rat thymus tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14027-1-AP targets ROD1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | ROD1 fusion protein Ag5172 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ROD1 regulator of differentiation 1 (S. pombe) |
| Synonyms | PTBP3, Regulator of differentiation 1, ROD1 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 55-59 kDa |
| GenBank Accession Number | BC044585 |
| Gene Symbol | ROD1 |
| Gene ID (NCBI) | 9991 |
| RRID | AB_2182321 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95758 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ROD1 is a RNA-binding protein that mediates pre-mRNA alternative splicing regulation, it plays a role in the regulation of cell proliferation, differentiation and migration. ROD1 exist some isoforms and also can be modified with the molecular weight range from 55-59 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ROD1 antibody 14027-1-AP | Download protocol |
| IHC protocol for ROD1 antibody 14027-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes.
| ||
iScience Polypyrimidine tract-binding protein 3/insulin-like growth factor 2 mRNA-binding proteins 3/high-mobility group A1 axis promotes renal cancer growth and metastasis | ||
Cancer Cell Int PTBP3 regulates proliferation of lung squamous cell carcinoma cells via CDC25A-mediated cell cycle progression. | ||
Cancer Cell Int PTBP3 promotes migration of non-small cell lung cancer through regulating E-cadherin in EMT signaling pathway. | ||
Oncol Lett Overexpression of ROD1 inhibits invasion of breast cancer cells by suppressing the translocation of β-catenin into the nucleus. | ||
Mol Cell PTBP1-activated co-transcriptional splicing controls epigenetic status of pluripotent stem cells |
