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ROPN1L Polyclonal antibody

ROPN1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12565-1-AP

Print datasheet

Synonyms

ropporin 1 like, ROPN1-like protein, ROPN1 like protein, ASP, AKAP-associated sperm protein

验证数据展示

  • WB analysis using 12565-1-AP

    WB analysis using 12565-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12565-1-AP (ROPN1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 12565-1-AP

    IHC staining of mouse testis using 12565-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12565-1-AP (ROPN1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 12565-1-AP

    IHC staining of mouse testis using 12565-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12565-1-AP (ROPN1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12565-1-AP

    IHC staining of human colon cancer using 12565-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12565-1-AP (ROPN1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12565-1-AP targets ROPN1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ROPN1L fusion protein Ag3178 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ropporin 1-like
Synonyms ropporin 1 like, ROPN1-like protein, ROPN1 like protein, ASP, AKAP-associated sperm protein
Calculated Molecular Weight 230 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC014607
Gene Symbol ROPN1L
Gene ID (NCBI) 83853
RRIDAB_10638785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96C74
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ROPN1L (Rhophilin Associated Tail Protein 1 Like) is a protein-coding gene that is a member of the ropporin family. ROPN1L plays a role in sperm function, as mutations in ROPN1L can cause defects in the fibrous sheath integrity, sperm motility, and PKA-dependent signaling processes, leading to male infertility.

实验方案

Product Specific Protocols
WB protocol for ROPN1L antibody 12565-1-APDownload protocol
IHC protocol for ROPN1L antibody 12565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols