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验证数据展示

  • WB analysis using 66923-1-Ig (same clone as 66923-1-PBS)

    WB analysis using 66923-1-Ig (same clone as 66923-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66923-1-Ig (same clone as 66923-1-PBS)

    IHC staining of human breast cancer using 66923-1-Ig (same clone as 66923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66923-1-Ig (ROR1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ROR1 antibody included
  • IHC staining of human breast cancer using 66923-1-Ig (same clone as 66923-1-PBS)

    IHC staining of human breast cancer using 66923-1-Ig (same clone as 66923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66923-1-Ig (ROR1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ROR1 antibody included
  • WB analysis of NIH/3T3 using 66923-1-Ig (same clone as 66923-1-PBS)

    WB analysis of NIH/3T3 using 66923-1-Ig (same clone as 66923-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51139-1

    Cytometric bead array standard curve of MP51139-1

    Cytometric bead array standard curve of MP51139-1, ROR1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66923-2-PBS. Detection antibody: 66923-1-PBS. Standard:Ag14586. Range: 0.391-100 ng/mL.

  • Cytometric bead array standard curve of MP51139-2

    Cytometric bead array standard curve of MP51139-2

    Cytometric bead array standard curve of MP51139-2, ROR1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66923-3-PBS. Detection antibody: 66923-1-PBS. Standard:Ag14586. Range: 0.781-100 ng/mL.

  • Featured Product
  • KD/KO验证

ROR1 Monoclonal antibody, PBS Only (Detector)

ROR1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66923-1-PBS

产品说明书

CloneNo. 1F5D8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

NTRKR1, Neurotrophic tyrosine kinase, receptor-related 1, Inactive tyrosine-protein kinase transmembrane receptor ROR1, 1F5D8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ROR1 Ready-To-Use IHC Kit. ROR1 primary antibody included.

产品信息

66923-1-PBS targets ROR1 as part of a matched antibody pair:

MP51139-1: 66923-2-PBS capture and 66923-1-PBS detection (validated in Cytometric bead array)

MP51139-2: 66923-3-PBS capture and 66923-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 ROR1 fusion protein Ag14586 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 receptor tyrosine kinase-like orphan receptor 1
别名 NTRKR1, Neurotrophic tyrosine kinase, receptor-related 1, Inactive tyrosine-protein kinase transmembrane receptor ROR1, 1F5D8
计算分子量 937 aa, 104 kDa
观测分子量 130 kDa
GenBank蛋白编号BC006374
基因名称 ROR1
Gene ID (NCBI) 4919
RRIDAB_2882250
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01973
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ROR1 is a member of the RTK family of orphan receptors related to muscle-specific kinase and Trk neurotrophin receptors (PMID: 18546292). ROR1 is primarily expressed by neural crest cells during embryogenesis. High expression of ROR1 is reported in many types of malignancies and is thought to be involved in tumor growth, apoptosis, and epithelial-mesenchymal transition (PMID: 26245996). The human ROR1 gene encodes a 937 amino acid protein with an Ig-like domain, a cysteine-rich domain, a kringle domain, a tyrosine kinase domain, and a proline-rich domain. The calculated molecular weight of ROR1 is 104 kDa, but ROR1 has multiple N-glycosylation sites that generate post-translationally modified ROR1 at 130 kDa (PMID: 24752542).