验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, HeLa cells, PC-3 cells, K-562 cells, Jurkat cells, L02 cells, SMMC-7721 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10616-1-AP targets RORA in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse , rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IHC, IP, ChIP |
| Tested Reactivity | human, mouse , rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | RORA fusion protein Ag0944 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | RAR-related orphan receptor A |
| Synonyms | NR1F1, Nuclear receptor ROR alpha, Nuclear receptor RZR alpha, RAR related orphan receptor A, RORA, RZR ALPHA, RZRA |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC008831 |
| Gene Symbol | RORA |
| Gene ID (NCBI) | 6095 |
| RRID | AB_2253844 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35398 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RORA antibody 10616-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med Hydrogen peroxide modulates clock gene expression via PRX2-STAT3-REV-ERBα/β pathway.
| ||
J Cell Physiol MCPIP1 regulates RORα expression to protect against liver injury induced by lipopolysaccharide via modulation of miR-155. | ||
Int Immunopharmacol ILC2s induce adaptive Th2-type immunity in different stages of tuberculosis through the Notch-GATA3 pathway. | ||
Biol Reprod Maternal melatonin or agomelatine therapy prevents programmed hypertension in male offspring of mother exposed to continuous light. |
