RORB Polyclonal antibody

RORB Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17635-1-AP

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Synonyms

NR1F2, Nuclear receptor ROR beta, Nuclear receptor RZR beta, RAR related orphan receptor B, ROR BETA, RORB, RZR BETA, RZRB



经过测试的应用

Positive WB detected inY79 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 3 publications below

产品信息

17635-1-AP targets RORB in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen RORB fusion protein Ag11910 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAR-related orphan receptor B
Synonyms NR1F2, Nuclear receptor ROR beta, Nuclear receptor RZR beta, RAR related orphan receptor B, ROR BETA, RORB, RZR BETA, RZRB
Calculated Molecular Weight 459 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC051830
Gene Symbol RORB
Gene ID (NCBI) 6096
RRIDAB_2180290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92753
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology [PMID:22189870]. In addition, NRs that lack identified ligands and are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family [PMID:7935491], which share a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone [PMID:17431400].

实验方案

Product Specific Protocols
WB protocol for RORB antibody 17635-1-APDownload protocol
IHC protocol for RORB antibody 17635-1-APDownload protocol
IP protocol for RORB antibody 17635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Neuron

Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia.

Authors - Chao Wang
mouseIF

PLoS Biol

An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold

Authors - Wenny Wong
mouseIF

eNeuro

Cellular and Behavioral Characterization of Pcdh19 Mutant Mice: subtle Molecular Changes, Increased Exploratory Behavior and an Impact of Social Environment.

Authors - Natalia Galindo-Riera