• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

Kazrin Polyclonal antibody

Kazrin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11572-1-AP

Print datasheet

Synonyms

KAZ, kazrin, KIAA1026, RP1 21O18.1, RP1-21O18.1

验证数据展示

  • WB analysis using 11572-1-AP

    WB analysis using 11572-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11572-1-AP

    IHC staining of human colon cancer using 11572-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11572-1-AP (kazrin antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with Kazrin antibody included

经过测试的应用

Positive WB detected inmouse skin tissue, mouse heart tissue, human heart tissue, mouse brain tissue, rat heart tissue, rat skin tissue
Positive IHC detected inhuman colon cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Kazrin Ready-To-Use IHC Kit. Kazrin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11572-1-AP targets Kazrin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Kazrin fusion protein Ag2152 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kazrin
Synonyms KAZ, kazrin, KIAA1026, RP1 21O18.1, RP1-21O18.1
Calculated Molecular Weight 415 aa, 46 kDa
Observed Molecular Weight 46 kDa, 37 kDa
GenBank Accession NumberBC035501
Gene Symbol Kazrin
Gene ID (NCBI) 23254
RRIDAB_833126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ674X7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kazrin is an evolutionarily conserved protein that is upregulated during keratinocyte terminal differentiation. Kazrin localizes to desmosomes and binds the epidermal cornified envelope protein periplakin. Kazrin exists three isoforms:kazrin A (46.7 kDa), kazrin B (46 kDa), and kazrin C (37 kDa).

实验方案

Product Specific Protocols
WB protocol for Kazrin antibody 11572-1-APDownload protocol
IHC protocol for Kazrin antibody 11572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

J Invest Dermatol

Exons 5-15 of kazrin are dispensable for murine epidermal morphogenesis and homeostasis.

Authors - Chhatriwala Mariya K MK
  • KD Validated
View Article