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DPCD Polyclonal antibody

DPCD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20936-1-AP

Print datasheet

Synonyms

DPCD, Protein DPCD, RP11 529I10.4, RP11-529I10.4

验证数据展示

  • WB analysis using 20936-1-AP

    WB analysis using 20936-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20936-1-AP (DPCD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 20936-1-AP

    IHC staining of mouse testis using 20936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20936-1-AP (DPCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 20936-1-AP

    IHC staining of mouse testis using 20936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20936-1-AP (DPCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20936-1-AP is DPCD Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DPCD fusion protein Ag15029 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deleted in a mouse model of primary ciliary dyskinesia
Synonyms DPCD, Protein DPCD, RP11 529I10.4, RP11-529I10.4
Calculated Molecular Weight 203 aa, 23 kDa
Observed Molecular Weight 23-26 kDa
GenBank Accession NumberBC031695
Gene Symbol DPCD
Gene ID (NCBI) 25911
RRIDAB_10734461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BVM2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DPCD (for deleted in a mouse model of primary ciliary dyskinesia), is predicted to code for a protein of 23 kDa which remains a novel candidate gene for PCD (Primary ciliary dyskinesia).

实验方案

Product Specific Protocols
WB protocol for DPCD antibody 20936-1-APDownload protocol
IHC protocol for DPCD antibody 20936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell Rep

DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling

Authors - Yu-Qian Mao
View Article
humanWB,IF

Sci Rep

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Authors - S Prpar Mihevc
View Article