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RP2 Polyclonal antibody

RP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14151-1-AP

Print datasheet

Synonyms

DELXp11.3, KIAA0215, Protein XRP2, RP2, TBCCD2

验证数据展示

  • WB analysis using 14151-1-AP

    WB analysis using 14151-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14151-1-AP (RP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 14151-1-AP

    IHC staining of human liver cancer using 14151-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14151-1-AP (RP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RP2 antibody included
  • IP experiment of Y79 using 14151-1-AP

    IP experiment of Y79 using 14151-1-AP

    IP result of anti-RP2 (IP:14151-1-AP, 4ug; Detection:14151-1-AP 1:500) with Y79 cells lysate 2000ug.

经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, Y79 cells, mouse liver tissue
Positive IP detected inY79 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RP2 Ready-To-Use IHC Kit. RP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14151-1-AP targets RP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen RP2 fusion protein Ag5332 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinitis pigmentosa 2 (X-linked recessive)
Synonyms DELXp11.3, KIAA0215, Protein XRP2, RP2, TBCCD2
Calculated Molecular Weight 350 aa, 40 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC043348
Gene Symbol RP2
Gene ID (NCBI) 6102
RRIDAB_11182501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75695
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RP2 antibody 14151-1-APDownload protocol
IHC protocol for RP2 antibody 14151-1-APDownload protocol
IP protocol for RP2 antibody 14151-1-APDownload protocol
FC protocol for RP2 antibody 14151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Cell Mol Life Sci

A look into retinal organoids: methods, analytical techniques, and applications.

Authors - Tess A V Afanasyeva
View Article
mouseWB

Sci Rep

The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein Golf to Olfactory Cilia.

Authors - Stefan Kurtenbach
View Article