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验证数据展示

  • IHC staining of human liver cancer using 14151-1-AP

    IHC staining of human liver cancer using 14151-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14151-1-AP (RP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RP2 antibody included
  • IP experiment of Y79 using 14151-1-AP

    IP experiment of Y79 using 14151-1-AP

    IP result of anti-RP2 (IP:14151-1-AP, 4ug; Detection:14151-1-AP 1:500) with Y79 cells lysate 2000ug.

RP2 Polyclonal antibody

RP2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14151-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

DELXp11.3, KIAA0215, Protein XRP2, RP2, TBCCD2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, Y79 cells, mouse liver tissue
Positive IP detected inY79 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RP2 Ready-To-Use IHC Kit. RP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14151-1-AP targets RP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 RP2 fusion protein Ag5332 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinitis pigmentosa 2 (X-linked recessive)
别名 DELXp11.3, KIAA0215, Protein XRP2, RP2, TBCCD2
计算分子量 350 aa, 40 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC043348
基因名称 RP2
Gene ID (NCBI) 6102
RRIDAB_11182501
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75695
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RP2 antibody 14151-1-APDownload protocol
IHC protocol for RP2 antibody 14151-1-APDownload protocol
IP protocol for RP2 antibody 14151-1-APDownload protocol
FC protocol for RP2 antibody 14151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Cell Mol Life Sci

A look into retinal organoids: methods, analytical techniques, and applications.

Authors - Tess A V Afanasyeva
View Article
mouseWB

Sci Rep

The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein Golf to Olfactory Cilia.

Authors - Stefan Kurtenbach
View Article