验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse spleen tissue |
Positive IP detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18144-1-AP targets RPA4 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IP, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | RPA4 fusion protein Ag12800 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | replication protein A4, 34kDa |
别名 | HSU24186, Replication factor A protein 4, replication protein A4, 34kDa, RF A protein 4, RP A p30, RPA4 |
计算分子量 | 261 aa, 29 kDa |
观测分子量 | 29-32 kDa |
GenBank蛋白编号 | BC069791 |
基因名称 | RPA4 |
Gene ID (NCBI) | 29935 |
RRID | AB_2180970 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q13156 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPA4, also named as RP-A p30, is a 261 amino acid protein, which belongs to the replication factor A protein 2 family. RPA4 is preferentially expressed in placental and colon mucosa. RPA4 as part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. This protein, Rpa4, has 47% identity with Rpa2, the 34-kDa subunit of RPA. (PMID: 7760808)
实验方案
Product Specific Protocols | |
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WB protocol for RPA4 antibody 18144-1-AP | Download protocol |
IP protocol for RPA4 antibody 18144-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |