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验证数据展示

  • WB analysis of HeLa using 18144-1-AP

    WB analysis of HeLa using 18144-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18144-1-AP (RPA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 18144-1-AP

    IP experiment of HeLa using 18144-1-AP

    IP result of anti-RPA4 (IP:18144-1-AP, 4ug; Detection:18144-1-AP 1:300) with HeLa cells lysate 3400ug.

RPA4 Polyclonal antibody

RPA4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 18144-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

HSU24186, Replication factor A protein 4, replication protein A4, 34kDa, RF A protein 4, RP A p30, RPA4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse spleen tissue
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18144-1-AP targets RPA4 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen RPA4 fusion protein Ag12800 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name replication protein A4, 34kDa
Synonyms HSU24186, Replication factor A protein 4, replication protein A4, 34kDa, RF A protein 4, RP A p30, RPA4
Calculated Molecular Weight 261 aa, 29 kDa
Observed Molecular Weight 29-32 kDa
GenBank Accession NumberBC069791
Gene Symbol RPA4
Gene ID (NCBI) 29935
RRIDAB_2180970
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13156
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPA4, also named as RP-A p30, is a 261 amino acid protein, which belongs to the replication factor A protein 2 family. RPA4 is preferentially expressed in placental and colon mucosa. RPA4 as part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. This protein, Rpa4, has 47% identity with Rpa2, the 34-kDa subunit of RPA. (PMID: 7760808)

实验方案

Product Specific Protocols
WB protocol for RPA4 antibody 18144-1-APDownload protocol
IP protocol for RPA4 antibody 18144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols