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RPAIN Polyclonal antibody

RPAIN Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 15220-1-AP

Print datasheet

Synonyms

HRIP, RIP, RPA interacting protein, RPAIN

验证数据展示

  • WB analysis of A2780 using 15220-1-AP

    WB analysis of A2780 using 15220-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of A375 using 15220-1-AP

    IP experiment of A375 using 15220-1-AP

    IP result of anti-RPAIN (IP:15220-1-AP, 4ug; Detection:15220-1-AP 1:300) with A375 cells lysate 2200 ug.

  • WB analysis of A375 using 15220-1-AP

    WB analysis of A375 using 15220-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 15220-1-AP

    IHC staining of human ovary tumor using 15220-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15220-1-AP (RPAIN antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary tumor using 15220-1-AP

    IHC staining of human ovary tumor using 15220-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15220-1-AP (RPAIN antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inA375 cells, mouse ovary tissue, A2780 cells
Positive IP detected inA375 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15220-1-AP targets RPAIN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB,ELISA Application Description
Tested Reactivity human, mouse
Immunogen RPAIN fusion protein Ag7392 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RPA interacting protein
Synonyms HRIP, RIP, RPA interacting protein, RPAIN
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 30-45 kDa
GenBank Accession NumberBC004451
Gene Symbol RPAIN
Gene ID (NCBI) 84268
RRIDAB_2180976
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UA6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAIN, also named as RIP, mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. RPAIN has many isoforms with MW 25-27 kDa, 12 kDa and 16-19 kDa. The publication with PMID: 16135809 reported the MW 30 and 45 kDa.

实验方案

Product Specific Protocols
WB protocol for RPAIN antibody 15220-1-APDownload protocol
IHC protocol for RPAIN antibody 15220-1-APDownload protocol
IP protocol for RPAIN antibody 15220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols