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RPAP3 Polyclonal antibody

RPAP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 23741-1-AP

Print datasheet

Synonyms

RNA polymerase II-associated protein 3

验证数据展示

  • WB analysis of HEK-293 using 23741-1-AP

    WB analysis of HEK-293 using 23741-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 23741-1-AP

    IHC staining of human stomach cancer using 23741-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPAP3 antibody included
  • IHC staining of human stomach cancer using 23741-1-AP

    IHC staining of human stomach cancer using 23741-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPAP3 antibody included
  • IP experiment of HEK-293 using 23741-1-AP

    IP experiment of HEK-293 using 23741-1-AP

    IP result of anti-RPAP3 (IP:23741-1-AP, 4ug; Detection:23741-1-AP 1:500) with HEK-293 cells lysate 2800ug.

  • IF Staining of HeLa using 23741-1-AP

    IF Staining of HeLa using 23741-1-AP

    Immunofluorescent analysis of HeLa cells using 23741-1-AP (RPAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPAP3 Ready-To-Use IHC Kit. RPAP3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

23741-1-AP targets RPAP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen RPAP3 fusion protein Ag20661 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA polymerase II associated protein 3
Synonyms RNA polymerase II-associated protein 3
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 76 kDa
GenBank Accession NumberBC056415
Gene Symbol RPAP3
Gene ID (NCBI) 79657
RRIDAB_2879313
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9H6T3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPAP3 antibody 23741-1-APDownload protocol
IHC protocol for RPAP3 antibody 23741-1-APDownload protocol
IF protocol for RPAP3 antibody 23741-1-APDownload protocol
IP protocol for RPAP3 antibody 23741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell Rep

DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling

Authors - Yu-Qian Mao
View Article
humanWB

Cell Chem Biol

RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.

Authors - Paul Yenerall
View Article
humanWB

Biochem Biophys Res Commun

HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma.

Authors - Akihiko Nakamura
View Article
humanWB

medRxiv

New ZNHIT3 Variants Disrupting snoRNP Assembly Cause Prenatal PEHO Syndrome with Isolated Hydrops

Authors - Md Lutfur Rahman
View Article