验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, mouse thymus tissue, HeLa cells, K-562 cells |
| Positive IF/ICC detected in | RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
12168-2-AP targets RPE in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | RPE fusion protein Ag2810 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribulose-5-phosphate-3-epimerase |
| 别名 | Ribulose-phosphate 3-epimerase, Ribulose-5-phosphate-3-epimerase, HUSSY-17, EC:5.1.3.1 |
| 计算分子量 | 228 aa, 25 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC016764 |
| 基因名称 | RPE |
| Gene ID (NCBI) | 6120 |
| RRID | AB_2253939 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96AT9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPE antibody 12168-2-AP | Download protocol |
| IF protocol for RPE antibody 12168-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep mTOR Overcomes Multiple Metabolic Restrictions to Enable HIV-1 Reverse Transcription and Intracellular Transport. | ||
Nat Metab Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions
|
