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RPGR Polyclonal antibody, PBS Only

RPGR Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16891-1-PBS

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Synonyms

验证数据展示

  • WB analysis of mouse eye using 16891-1-AP (same clone as 16891-1-PBS)

    WB analysis of mouse eye using 16891-1-AP (same clone as 16891-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 cells using 16891-1-AP (same clone as 16891-1-PBS)

    IF Staining of hTERT-RPE1 cells using 16891-1-AP (same clone as 16891-1-PBS)

    IF result of anti-RPGR (16891-1-AP) in hTERT-RPE1 cell by Dr. Seongjin Seo. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 16891-1-AP (same clone as 16891-1-PBS)

    IHC staining of mouse eye using 16891-1-AP (same clone as 16891-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 16891-1-AP (RPGR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 16891-1-AP (same clone as 16891-1-PBS)

    IHC staining of mouse eye using 16891-1-AP (same clone as 16891-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 16891-1-AP (RPGR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 16891-1-AP (same clone as 16891-1-PBS)

    IHC staining of human kidney using 16891-1-AP (same clone as 16891-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

产品信息

16891-1-PBS targets RPGR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen RPGR fusion protein Ag10405 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinitis pigmentosa GTPase regulator
Synonyms
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 100-105 kDa, 70 kDa
GenBank Accession NumberBC031624
Gene Symbol RPGR
Gene ID (NCBI) 6103
RRIDAB_2181130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92834
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPGR is a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined. This antibody can recognize all the isoforms of RPGR.