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验证数据展示

  • WB analysis using 13010-1-AP

    WB analysis using 13010-1-AP

    mouse spleen tissues were subjected to SDS PAGE followed by western blot with 13010-1-AP (RPIA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 13010-1-AP

    IF Staining of HepG2 using 13010-1-AP

    Immunofluorescent analysis of HepG2 cells, using RPIA antibody 13010-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • WB analysis using 13010-1-AP

    WB analysis using 13010-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13010-1-AP (RPIA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

RPIA Polyclonal antibody

RPIA Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13010-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (1)

别名

RPI, Ribose-5-phosphate isomerase, ribose 5 phosphate isomerase A, Phosphoriboisomerase, EC:5.3.1.6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue, HEK-293 cells, MCF-7 cells, PC-3 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13010-1-AP targets RPIA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 RPIA fusion protein Ag3699 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribose 5-phosphate isomerase A
别名 RPI, Ribose-5-phosphate isomerase, ribose 5 phosphate isomerase A, Phosphoriboisomerase, EC:5.3.1.6
计算分子量 311 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC015529
基因名称 RPIA
Gene ID (NCBI) 22934
RRIDAB_10638301
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49247
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPIA(Ribose-5-phosphate isomerase) is also named as RPI and belongs to the ribose 5-phosphate isomerase family. It is involved in pentose-phosphate shunt, non-oxidative branch. It is an enzyme in the pentose-phosphate pathway, appears to be determined by a single structural locus and is probably a dimer(PMID:7396409).

实验方案

Product Specific Protocols
WB protocol for RPIA antibody 13010-1-APDownload protocol
IF protocol for RPIA antibody 13010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Int J Biol Sci

Diallyl disulfide induces DNA damage and growth inhibition in colorectal cancer cells by promoting POU2F1 ubiquitination

Authors - Longzheng Xia
View Article