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验证数据展示

  • WB analysis using 68110-1-Ig

    WB analysis using 68110-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68110-1-Ig (RPL12 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human placenta using 68110-1-Ig

    IHC staining of human placenta using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included
  • IHC staining of human endometrial cancer using 68110-1-Ig

    IHC staining of human endometrial cancer using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included
  • IHC staining of mouse brain using 68110-1-Ig

    IHC staining of mouse brain using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included
  • IHC staining of mouse stomach using 68110-1-Ig

    IHC staining of mouse stomach using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included
  • IHC staining of rat brain using 68110-1-Ig

    IHC staining of rat brain using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included
  • IHC staining of rat stomach using 68110-1-Ig

    IHC staining of rat stomach using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included

RPL12 Monoclonal antibody

RPL12 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68110-1-Ig

产品说明书

CloneNo. 2C9B3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ribosomal protein L12, Large ribosomal subunit protein uL11, 60S ribosomal protein L12, 2C9B3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, human endometrial cancer tissue, human placenta tissue, mouse stomach tissue, rat brain tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RPL12 Ready-To-Use IHC Kit. RPL12 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:8000-1:32000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68110-1-Ig targets RPL12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPL12 fusion protein Ag6580 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein L12
别名 ribosomal protein L12, Large ribosomal subunit protein uL11, 60S ribosomal protein L12, 2C9B3
计算分子量 18 kDa
观测分子量21 kDa
GenBank蛋白编号BC050644
基因名称 RPL12
Gene ID (NCBI) 6136
RRIDAB_2923639
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP30050
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL12 antibody 68110-1-IgDownload protocol
IHC protocol for RPL12 antibody 68110-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols