验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, SGC-7901 cells, human lung tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RPL13 Ready-To-Use IHC Kit. RPL13 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
11271-1-AP targets RPL13 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RPL13 fusion protein Ag1814 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L13 |
| 别名 | 60S ribosomal protein L13, BBC1, D16S444E, ribosomal protein L13, RPL13 |
| 计算分子量 | 24 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC014167 |
| 基因名称 | RPL13 |
| Gene ID (NCBI) | 6137 |
| RRID | AB_2269735 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P26373 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL13 may be a plausible candidate tumor suppressor gene, as knocking down L13 expression using small interfering RNA (siRNA) resulted in drastic attenuation of cancer cell growth with significant G1 and G2/M arrest of the cell cycle[PMID:16786168].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL13 antibody 11271-1-AP | Download protocol |
| IHC protocol for RPL13 antibody 11271-1-AP | Download protocol |
| IP protocol for RPL13 antibody 11271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response. | ||
Mol Cell Proteomics Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer. | ||
Ecotoxicol Environ Saf ZEA and DON inhibited inflammation after L. monocytogenes infection and induced ribosomal hyperfunction. |
