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RPL14 Polyclonal antibody

RPL14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14991-1-AP

Print datasheet

Synonyms

Large ribosomal subunit protein eL14, L14, hRL14, CTG B33, CAG-ISL 7

验证数据展示

  • WB analysis using 14991-1-AP

    WB analysis using 14991-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 14991-1-AP

    IHC staining of human colon cancer using 14991-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14991-1-AP (RPL14 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with RPL14 antibody included
  • IHC staining of human colon cancer using 14991-1-AP

    IHC staining of human colon cancer using 14991-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14991-1-AP (RPL14 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with RPL14 antibody included
  • IF Staining of HepG2 using 14991-1-AP

    IF Staining of HepG2 using 14991-1-AP

    Immunofluorescent analysis of HepG2 cells, using RPL14 antibody 14991-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of mouse liver using 14991-1-AP

    IP experiment of mouse liver using 14991-1-AP

    IP result of anti-RPL14 (IP:14991-1-AP, 4ug; Detection:14991-1-AP 1:1000) with mouse liver tissue lysate 6800ug.

  • WB analysis using 14991-1-AP

    WB analysis using 14991-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPL14 Ready-To-Use IHC Kit. RPL14 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14991-1-AP targets RPL14 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, CoIP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RPL14 fusion protein Ag6976 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L14
Synonyms Large ribosomal subunit protein eL14, L14, hRL14, CTG B33, CAG-ISL 7
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC000606
Gene Symbol RPL14
Gene ID (NCBI) 9045
RRIDAB_2285319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50914
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPL14 is a component of the large ribosomal subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain.

实验方案

Product Specific Protocols
WB protocol for RPL14 antibody 14991-1-APDownload protocol
IHC protocol for RPL14 antibody 14991-1-APDownload protocol
IF protocol for RPL14 antibody 14991-1-APDownload protocol
IP protocol for RPL14 antibody 14991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Res

Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.

Authors - Xiaochen Gai
View Article
humanWB,IF

Cell Discov

Ribosomal RNA regulates chromosome clustering during mitosis.

Authors - Kai Ma
View Article
humanWB

Am J Cancer Res

UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc

Authors - Dalian Gong
View Article
humanCoIP,WB

Cell Discov

The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination

Authors - Duo Wu
View Article