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验证数据展示

  • WB analysis using 83928-1-RR

    WB analysis using 83928-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83928-1-RR (RPL18A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83928-1-RR

    IHC staining of human stomach cancer using 83928-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83928-1-RR (RPL18A antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Affinity and Kinetic Characterization of 83928-1-RR

    Affinity and Kinetic Characterization of 83928-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83928-1-RR against Human RPL18A were performed. The affinity constant is below 1 pM.

RPL18A Recombinant antibody

RPL18A Recombinant Antibody for WB, IHC, ELISA
Cat No. 83928-1-RR

产品说明书

CloneNo. 240841B1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ribosomal protein L18a, Large ribosomal subunit protein eL20, 60S ribosomal protein L18a, 240841B1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells, MCF-7 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83928-1-RR targets RPL18A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPL18A fusion protein Ag6251 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein L18a
别名 ribosomal protein L18a, Large ribosomal subunit protein eL20, 60S ribosomal protein L18a, 240841B1
计算分子量 21 kDa
观测分子量 23 kDa
GenBank蛋白编号BC066319
基因名称 RPL18A
Gene ID (NCBI) 6142
RRIDAB_3671508
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ02543
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPL18A, also named as 60S ribosomal protein L18a(Ribosomal Protein L18a), located on 19p13.11, is a protein coding gene. RPL18A is a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). Diseases such as Hepatitis C Virus and Omsk Hemorrhagic Fever are associated with RPL18A. The molecular weight of RPL18A is 23 kDa.

实验方案

Product Specific Protocols
WB protocol for RPL18A antibody 83928-1-RRDownload protocol
IHC protocol for RPL18A antibody 83928-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols