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RPL21 Polyclonal antibody

RPL21 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15226-1-AP

Print datasheet

Synonyms

60S ribosomal protein L21, L21, ribosomal protein L21, RPL21

验证数据展示

  • WB analysis of HepG2 using 15226-1-AP

    WB analysis of HepG2 using 15226-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15226-1-AP (RPL21 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15226-1-AP

    IHC staining of human liver using 15226-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15226-1-AP (RPL21 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with RPL21 antibody included
  • IHC staining of human liver using 15226-1-AP

    IHC staining of human liver using 15226-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15226-1-AP (RPL21 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with RPL21 antibody included

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RPL21 Ready-To-Use IHC Kit. RPL21 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15226-1-AP targets RPL21 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RPL21 fusion protein Ag7444 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L21
Synonyms 60S ribosomal protein L21, L21, ribosomal protein L21, RPL21
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC001603
Gene Symbol RPL21
Gene ID (NCBI) 6144
RRIDAB_2181591
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46778
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL21 antibody 15226-1-APDownload protocol
IHC protocol for RPL21 antibody 15226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment

Authors - Magdalena Wolczyk
View Article