• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

RPL27A Polyclonal antibody

RPL27A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16002-1-AP

Print datasheet

Synonyms

ribosomal protein L27a, Large ribosomal subunit protein uL15, 60S ribosomal protein L27a

验证数据展示

  • WB analysis of A549 using 16002-1-AP

    WB analysis of A549 using 16002-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16002-1-AP (RPL27A Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IF Staining of A549 using 16002-1-AP

    IF Staining of A549 using 16002-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 16002-1-AP (RPL27A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse stomach using 16002-1-AP

    IHC staining of mouse stomach using 16002-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16002-1-AP (RPL27A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 16002-1-AP

    IHC staining of mouse stomach using 16002-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16002-1-AP (RPL27A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16002-1-AP targets RPL27A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RPL27A fusion protein Ag8596 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L27a
Synonyms ribosomal protein L27a, Large ribosomal subunit protein uL15, 60S ribosomal protein L27a
Calculated Molecular Weight 148 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC005326
Gene Symbol RPL27A
Gene ID (NCBI) 6157
RRIDAB_2878206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46776
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL27A antibody 16002-1-APDownload protocol
IHC protocol for RPL27A antibody 16002-1-APDownload protocol
IF protocol for RPL27A antibody 16002-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,FC

Transl Res

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Authors - Mahmoud Dabbah
View Article
humanWB

Bioengineered

tRF-19-W4PU732S promotes breast cancer cell malignant activity by targeting inhibition of RPL27A (ribosomal protein-L27A).

Authors - Zhengxiang Zhang
  • KD Validated
View Article