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RPL29 Recombinant antibody, PBS Only

RPL29 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240062F9

Cat no : 83377-1-PBS

Print datasheet

Synonyms

Large ribosomal subunit protein eL29, HUMRPL29, HIP, Cell surface heparin-binding protein HIP, 60S ribosomal protein L29

验证数据展示

  • WB analysis using 83377-1-RR (same clone as 83377-1-PBS)

    WB analysis using 83377-1-RR (same clone as 83377-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83377-1-RR (RPL29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

    IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

    IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83377-1-RR (same clone as 83377-1-PBS)

    FC experiment of HeLa using 83377-1-RR (same clone as 83377-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPL29 Recombinant antibody (83377-1-RR, Clone:240062F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83377-1-RR

    Affinity and Kinetic Characterization of 83377-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83377-1-RR against Human RPL29 were performed. The affinity constant is 0.254 nM.

产品信息

83377-1-PBS targets RPL29 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RPL29 fusion protein Ag34100 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein L29
Synonyms Large ribosomal subunit protein eL29, HUMRPL29, HIP, Cell surface heparin-binding protein HIP, 60S ribosomal protein L29
Calculated Molecular Weight 159 aa, 18 kDa
GenBank Accession NumberBC008926
Gene Symbol RPL29
Gene ID (NCBI) 6159
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP47914
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C