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验证数据展示

  • WB analysis using 83377-2-RR

    WB analysis using 83377-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83377-2-RR (RPL29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83377-2-RR

    IF Staining of HepG2 using 83377-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-2-RR, Clone: 240062A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83377-2-RR

    Affinity and Kinetic Characterization of 83377-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83377-2-RR against Human RPL29 were performed. The affinity constant is 1.04 nM.

RPL29 Recombinant antibody

RPL29 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83377-2-RR

产品说明书

CloneNo. 240062A3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Large ribosomal subunit protein eL29, HUMRPL29, HIP, Cell surface heparin-binding protein HIP, 60S ribosomal protein L29

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells, U-87 MG cells, C6 cells, NIH/3T3 cells,
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83377-2-RR targets RPL29 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPL29 fusion protein Ag34100 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein L29
别名 Large ribosomal subunit protein eL29, HUMRPL29, HIP, Cell surface heparin-binding protein HIP, 60S ribosomal protein L29
计算分子量 159 aa, 18 kDa
观测分子量 20-25 kDa
GenBank蛋白编号BC008926
基因名称 RPL29
Gene ID (NCBI) 6159
RRIDAB_3671031
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP47914
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL29 antibody 83377-2-RRDownload protocol
IF protocol for RPL29 antibody 83377-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols