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  • Featured Product
  • KD/KO Validated

RPL3 Polyclonal antibody

RPL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11005-1-AP

Print datasheet

Synonyms

TARBP B, ribosomal protein L3, OK/SW-cl.32, Large ribosomal subunit protein uL3, HIV-1 TAR RNA-binding protein B

验证数据展示

  • WB analysis of HeLa using 11005-1-AP

    WB analysis of HeLa using 11005-1-AP

    WB result of RPL3 antibody (11005-1-AP, 1:2000) with si-control and si-RPL3 transfected HeLa cells..

  • WB analysis using 11005-1-AP

    WB analysis using 11005-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11005-1-AP (RPL3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 11005-1-AP

    IP experiment of mouse kidney using 11005-1-AP

    IP result of anti-RPL3 (IP:11005-1-AP, 4ug; Detection:11005-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

  • IF Staining of HepG2 using 11005-1-AP

    IF Staining of HepG2 using 11005-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL3 antibody (11005-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human prostate cancer using 11005-1-AP

    IHC staining of human prostate cancer using 11005-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11005-1-AP (RPL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, human kidney tissue, HeLa cells, Raji cells, human placenta tissue, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11005-1-AP targets RPL3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen RPL3 fusion protein Ag1436 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L3
Synonyms TARBP B, ribosomal protein L3, OK/SW-cl.32, Large ribosomal subunit protein uL3, HIV-1 TAR RNA-binding protein B
Calculated Molecular Weight 46/27 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC012786
Gene Symbol RPL3
Gene ID (NCBI) 6122
RRIDAB_2181760
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP39023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. This is a rabbit polyclonal antibody raised against the C terminus of human RPL3.

实验方案

Product Specific Protocols
WB protocol for RPL3 antibody 11005-1-APDownload protocol
IHC protocol for RPL3 antibody 11005-1-APDownload protocol
IF protocol for RPL3 antibody 11005-1-APDownload protocol
IP protocol for RPL3 antibody 11005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein.

Authors - Elizabeth I Vink
  • KD Validated
View Article
humanWB

Elife

METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.

Authors - Eriko Matsuura-Suzuki
View Article
humanWB

Cell Discov

Ribosomal RNA regulates chromosome clustering during mitosis.

Authors - Kai Ma
View Article
mouseWB

Aging (Albany NY)

RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation.

Authors - Chan Chen
View Article
humanWB

Nucleic Acid Ther

Knockdown of Muscle-Specific Ribosomal Protein L3-Like Enhances Muscle Function in Healthy and Dystrophic Mice.

Authors - Betty R Kao
View Article
humanIHC

Carcinogenesis

DUOX2 promotes the progression of colorectal cancer cells by regulating the AKT pathway and interacting with RPL3.

Authors - Xue Zhang
View Article