• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RPL34 Polyclonal antibody

RPL34 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15179-1-AP

Print datasheet

Synonyms

ribosomal protein L34, Large ribosomal subunit protein eL34, 60S ribosomal protein L34

验证数据展示

  • WB analysis using 15179-1-AP

    WB analysis using 15179-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15179-1-AP (RPL34 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 15179-1-AP

    IF Staining of A431 using 15179-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RPL34 antibody (15179-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse pancreas using 15179-1-AP

    IHC staining of mouse pancreas using 15179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15179-1-AP (RPL34 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL34 antibody included

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells, mouse liver tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy RPL34 Ready-To-Use IHC Kit. RPL34 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15179-1-AP targets RPL34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RPL34 fusion protein Ag7100 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L34
Synonyms ribosomal protein L34, Large ribosomal subunit protein eL34, 60S ribosomal protein L34
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC001773
Gene Symbol RPL34
Gene ID (NCBI) 6164
RRIDAB_3085450
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49207
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL34 antibody 15179-1-APDownload protocol
IHC protocol for RPL34 antibody 15179-1-APDownload protocol
IF protocol for RPL34 antibody 15179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols