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验证数据展示

  • WB analysis using 15179-1-AP

    WB analysis using 15179-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15179-1-AP (RPL34 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 15179-1-AP

    IF Staining of A431 using 15179-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RPL34 antibody (15179-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse pancreas using 15179-1-AP

    IHC staining of mouse pancreas using 15179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15179-1-AP (RPL34 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL34 antibody included

RPL34 Polyclonal antibody

RPL34 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15179-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ribosomal protein L34, Large ribosomal subunit protein eL34, 60S ribosomal protein L34

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells, mouse liver tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy RPL34 Ready-To-Use IHC Kit. RPL34 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15179-1-AP targets RPL34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPL34 fusion protein Ag7100 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein L34
别名 ribosomal protein L34, Large ribosomal subunit protein eL34, 60S ribosomal protein L34
计算分子量 13 kDa
观测分子量 13 kDa
GenBank蛋白编号BC001773
基因名称 RPL34
Gene ID (NCBI) 6164
RRIDAB_3085450
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49207
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL34 antibody 15179-1-APDownload protocol
IHC protocol for RPL34 antibody 15179-1-APDownload protocol
IF protocol for RPL34 antibody 15179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols