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RPL35 Polyclonal antibody

RPL35 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14826-1-AP

Print datasheet

Synonyms

ribosomal protein L35, Large ribosomal subunit protein uL29, 60S ribosomal protein L35

验证数据展示

  • WB analysis of mouse heart using 14826-1-AP

    WB analysis of mouse heart using 14826-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IF Staining of HeLa using 14826-1-AP

    IF Staining of HeLa using 14826-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14826-1-AP (RPL35 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 14826-1-AP

    IHC staining of human liver cancer using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included
  • IHC staining of human liver cancer using 14826-1-AP

    IHC staining of human liver cancer using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included
  • IHC staining of human pancreas cancer using 14826-1-AP

    IHC staining of human pancreas cancer using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included
  • IHC staining of human pancreas cancer using 14826-1-AP

    IHC staining of human pancreas cancer using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included
  • IHC staining of human stomach cancer using 14826-1-AP

    IHC staining of human stomach cancer using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included
  • IHC staining of mouse pancreas using 14826-1-AP

    IHC staining of mouse pancreas using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included
  • IHC staining of rat pancreas using 14826-1-AP

    IHC staining of rat pancreas using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included

经过测试的应用

Positive WB detected inmouse heart tissue, HeLa cells, mouse skeletal muscle tissue
Positive IHC detected inrat pancreas tissue, human liver cancer tissue, human pancreas cancer tissue, human stomach cancer tissue, human thyroid cancer tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPL35 Ready-To-Use IHC Kit. RPL35 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14826-1-AP targets RPL35 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen RPL35 fusion protein Ag6615 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L35
Synonyms ribosomal protein L35, Large ribosomal subunit protein uL29, 60S ribosomal protein L35
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC000348
Gene Symbol RPL35
Gene ID (NCBI) 11224
RRIDAB_2878083
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42766
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL35 antibody 14826-1-APDownload protocol
IHC protocol for RPL35 antibody 14826-1-APDownload protocol
IF protocol for RPL35 antibody 14826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Cell Sci

Drp1 regulates transcription of ribosomal protein genes in embryonic hearts.

Authors - Qiancong Zhao
View Article