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验证数据展示

  • WB analysis of PC-12 using 15145-1-AP

    WB analysis of PC-12 using 15145-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15145-1-AP

    IF Staining of HeLa using 15145-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPL36 antibody (15145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RPL36 Polyclonal antibody

RPL36 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15145-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ribosomal protein L36, Large ribosomal subunit protein eL36, 60S ribosomal protein L36

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-12 cells, BxPC-3 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15145-1-AP targets RPL36 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 RPL36 fusion protein Ag7437 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein L36
别名 ribosomal protein L36, Large ribosomal subunit protein eL36, 60S ribosomal protein L36
计算分子量 12 kDa
观测分子量 12-14 kDa
GenBank蛋白编号BC003052
基因名称 RPL36
Gene ID (NCBI) 25873
RRIDAB_2878111
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3U8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPL36, also named as 60S ribosomal protein L36, is a 105 amino acid protein, which is a component of the large ribosomal subunit. RPL36 may be involved in the early stage of hepatocarcinogenesis, and it can be used as an independent and potential prognostic marker for resected HCC.

实验方案

Product Specific Protocols
WB protocol for RPL36 antibody 15145-1-APDownload protocol
IF protocol for RPL36 antibody 15145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

METTL3 preferentially enhances non-m6A translation of epigenetic factors and promotes tumourigenesis

Authors - Xueju Wei
View Article
mouseWB

J Pineal Res

Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin

Authors - Jingming Ren
View Article