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RPL36 Polyclonal antibody

RPL36 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15145-1-AP

Print datasheet

Synonyms

ribosomal protein L36, Large ribosomal subunit protein eL36, 60S ribosomal protein L36

验证数据展示

  • WB analysis of PC-12 using 15145-1-AP

    WB analysis of PC-12 using 15145-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15145-1-AP

    IF Staining of HeLa using 15145-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPL36 antibody (15145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inPC-12 cells, BxPC-3 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15145-1-AP targets RPL36 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RPL36 fusion protein Ag7437 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L36
Synonyms ribosomal protein L36, Large ribosomal subunit protein eL36, 60S ribosomal protein L36
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12-14 kDa
GenBank Accession NumberBC003052
Gene Symbol RPL36
Gene ID (NCBI) 25873
RRIDAB_2878111
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3U8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPL36, also named as 60S ribosomal protein L36, is a 105 amino acid protein, which is a component of the large ribosomal subunit. RPL36 may be involved in the early stage of hepatocarcinogenesis, and it can be used as an independent and potential prognostic marker for resected HCC.

实验方案

Product Specific Protocols
WB protocol for RPL36 antibody 15145-1-APDownload protocol
IF protocol for RPL36 antibody 15145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

METTL3 preferentially enhances non-m6A translation of epigenetic factors and promotes tumourigenesis

Authors - Xueju Wei
View Article
mouseWB

J Pineal Res

Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin

Authors - Jingming Ren
View Article