验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, PC-12 cells, RAW 264.7 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15185-1-AP targets RPL36A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | RPL36A fusion protein Ag7210 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ribosomal protein L36a |
别名 | MIG6, Large ribosomal subunit protein eL42, L44L, GIG15, Cell migration-inducing gene 6 protein |
计算分子量 | 12 kDa |
观测分子量 | 12-17 kDa |
GenBank蛋白编号 | BC001781 |
基因名称 | RPL36A |
Gene ID (NCBI) | 6173 |
RRID | AB_3669200 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P83881 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for RPL36A antibody 15185-1-AP | Download protocol |
IHC protocol for RPL36A antibody 15185-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |