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验证数据展示

  • WB analysis of HeLa using 15055-1-AP

    WB analysis of HeLa using 15055-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15055-1-AP (RPL38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 15055-1-AP

    IHC staining of human pancreas cancer using 15055-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15055-1-AP (RPL38 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas cancer using 15055-1-AP

    IHC staining of human pancreas cancer using 15055-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15055-1-AP (RPL38 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 15055-1-AP

    IF Staining of MCF-7 using 15055-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 15055-1-AP (RPL38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RPL38 Polyclonal antibody

RPL38 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15055-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ribosomal protein L38, Large ribosomal subunit protein eL38, 60S ribosomal protein L38

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15055-1-AP targets RPL38 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPL38 fusion protein Ag7038 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein L38
别名 ribosomal protein L38, Large ribosomal subunit protein eL38, 60S ribosomal protein L38
计算分子量 8 kDa
观测分子量 8 kDa
GenBank蛋白编号BC000603
基因名称 RPL38
Gene ID (NCBI) 6169
RRIDAB_10858918
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP63173
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL38 antibody 15055-1-APDownload protocol
IHC protocol for RPL38 antibody 15055-1-APDownload protocol
IF protocol for RPL38 antibody 15055-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Oncol Rep

PP2A inhibitors suppress migration and growth of PANC-1 pancreatic cancer cells through inhibition on the Wnt/β-catenin pathway by phosphorylation and degradation of β-catenin.

Authors - Meng-Yao Wu
View Article