验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14990-1-AP targets RPL39 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, zebrafish |
| 免疫原 | RPL39 fusion protein Ag6975 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L39 |
| 别名 | ribosomal protein L39, Large ribosomal subunit protein eL39, eL39, 60S ribosomal protein L39 |
| 计算分子量 | 6 kDa |
| 观测分子量 | 6 kDa |
| GenBank蛋白编号 | BC001019 |
| 基因名称 | RPL39 |
| Gene ID (NCBI) | 6170 |
| RRID | AB_2238670 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62891 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL39 antibody 14990-1-AP | Download protocol |
| IHC protocol for RPL39 antibody 14990-1-AP | Download protocol |
| IF protocol for RPL39 antibody 14990-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Res Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication. | ||
Elife In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration. | ||
Front Immunol Identification and validation of a 17-gene signature to improve the survival prediction of gliomas
| ||
iScience Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.
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