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RPL8 Polyclonal antibody

RPL8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16981-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

ribosomal protein L8, Large ribosomal subunit protein uL2, 60S ribosomal protein L8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16981-1-AP targets RPL8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RPL8 fusion protein Ag9957 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L8
Synonyms ribosomal protein L8, Large ribosomal subunit protein uL2, 60S ribosomal protein L8
Calculated Molecular Weight 257 aa, 28 kDa
Observed Molecular Weight 28-34 kDa
GenBank Accession NumberBC012197
Gene Symbol RPL8
Gene ID (NCBI) 6132
RRIDAB_2182071
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62917
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL8 antibody 16981-1-APDownload protocol
IHC protocol for RPL8 antibody 16981-1-APDownload protocol
IF protocol for RPL8 antibody 16981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages

Authors - Teodorus Theo Susanto
humanIF

Cell Discov

Ribosomal RNA regulates chromosome clustering during mitosis.

Authors - Kai Ma
humanWB

Genes (Basel)

Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.

Authors - Meijuan Zou
  • KD Validated

Nucleic Acids Res

ADAR3 modulates neuronal differentiation and regulates mRNA stability and translation

Authors - Victor Karlström
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