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RPL8 Polyclonal antibody

RPL8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16981-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

ribosomal protein L8, Large ribosomal subunit protein uL2, 60S ribosomal protein L8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16981-1-AP targets RPL8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 RPL8 fusion protein Ag9957 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein L8
别名 ribosomal protein L8, Large ribosomal subunit protein uL2, 60S ribosomal protein L8
计算分子量 257 aa, 28 kDa
观测分子量 28-34 kDa
GenBank蛋白编号BC012197
基因名称 RPL8
Gene ID (NCBI) 6132
RRIDAB_2182071
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62917
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL8 antibody 16981-1-APDownload protocol
IHC protocol for RPL8 antibody 16981-1-APDownload protocol
IF protocol for RPL8 antibody 16981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages

Authors - Teodorus Theo Susanto
humanIF

Cell Discov

Ribosomal RNA regulates chromosome clustering during mitosis.

Authors - Kai Ma
humanWB

Genes (Basel)

Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.

Authors - Meijuan Zou
  • KD Validated

Nucleic Acids Res

ADAR3 modulates neuronal differentiation and regulates mRNA stability and translation

Authors - Victor Karlström
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