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验证数据展示

  • WB analysis using 68054-1-Ig

    WB analysis using 68054-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68054-1-Ig (RPL9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of mouse cerebellum using 68054-1-Ig

    IF Staining of mouse cerebellum using 68054-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse cerebellum using 68054-1-Ig

    IHC staining of mouse cerebellum using 68054-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 68054-1-Ig

    IHC staining of mouse cerebellum using 68054-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RPL9 Monoclonal antibody

RPL9 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 68054-1-Ig

产品说明书

CloneNo. 2A6B11
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

2A6B11, 60S ribosomal protein L9, FLJ27456, Large ribosomal subunit protein uL6, OK/SW-cl.103

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, A549 cells, HepG2 cells, MCF-7 cells, U2OS cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68054-1-Ig targets RPL9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原 RPL9 fusion protein Ag5391 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein L9
别名 2A6B11, 60S ribosomal protein L9, FLJ27456, Large ribosomal subunit protein uL6, OK/SW-cl.103
计算分子量 22 kDa
观测分子量22 kDa
GenBank蛋白编号BC066318
基因名称 RPL9
Gene ID (NCBI) 6133
RRIDAB_2918795
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP32969
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL9 antibody 68054-1-IgDownload protocol
IHC protocol for RPL9 antibody 68054-1-IgDownload protocol
IF protocol for RPL9 antibody 68054-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

J Cardiovasc Transl Res

CREB1 Silencing Protects Against Inflammatory Response in Rats with Deep Vein Thrombosis Through Reducing RPL9 Expression and Blocking NF-κB Signaling

Authors - Xiaorong Jian
View Article