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  • KD/KO Validated

RPLP1 Polyclonal antibody

RPLP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 21636-1-AP

Print datasheet

Synonyms

P1, ribosomal protein, large, P1, RPLP1, RPP1, RRP1



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPLP1 Ready-To-Use IHC Kit. RPLP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21636-1-AP targets RPLP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Immunogen RPLP1 fusion protein Ag16268 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein, large, P1
Synonyms P1, ribosomal protein, large, P1, RPLP1, RPP1, RRP1
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 15-20 kDa
GenBank Accession NumberBC003369
Gene Symbol RPLP1
Gene ID (NCBI) 6176
RRIDAB_2878894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05386
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

60S acidic ribosomal protein P1 (RRP1) is a 114 amino acid protein, which belongs to the ribosomal protein L12P family. RRP1 plays an important role in the elongation step of protein synthesis. The molecular weight of modified RRP1 is 15-20 kDa.

实验方案

Product Specific Protocols
WB protocol for RPLP1 antibody 21636-1-APDownload protocol
IHC protocol for RPLP1 antibody 21636-1-APDownload protocol
IF protocol for RPLP1 antibody 21636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Virulence

RPLP1, an NS4B-interacting protein, enhances production of CSFV through promoting translation of viral genome.

Authors - Longxiang Zhang
  • KD Validated
mouseWB

Mol Hum Reprod

60S acidic ribosomal protein P1 (RPLP1) is elevated in human endometriotic tissue and in a murine model of endometriosis and is essential for endometriotic epithelial cell survival in vitro.

Authors - Zahraa Alali
humanWB

Int J Mol Sci

RPLP1 Is Up-Regulated in Human Adenomyosis and Endometrial Adenocarcinoma Epithelial Cells and Is Essential for Cell Survival and Migration In Vitro

Authors - Riley Peterson
humanWB,IHC

Am J Cancer Res

Unveiling the prognostic implications of RPLP1 upregulation in osteosarcoma

Authors - Xinhui Du
humanWB,IF

Nat Commun

RPLP1 restricts HIV-1 transcription by disrupting C/EBPβ binding to the LTR

Authors - Weijing Yang