验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy RPLP1 Ready-To-Use IHC Kit. RPLP1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21636-1-AP targets RPLP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Immunogen | RPLP1 fusion protein Ag16268 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ribosomal protein, large, P1 |
| Synonyms | P1, ribosomal protein, large, P1, RPLP1, RPP1, RRP1 |
| Calculated Molecular Weight | 12 kDa |
| Observed Molecular Weight | 15-20 kDa |
| GenBank Accession Number | BC003369 |
| Gene Symbol | RPLP1 |
| Gene ID (NCBI) | 6176 |
| RRID | AB_2878894 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05386 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
60S acidic ribosomal protein P1 (RRP1) is a 114 amino acid protein, which belongs to the ribosomal protein L12P family. RRP1 plays an important role in the elongation step of protein synthesis. The molecular weight of modified RRP1 is 15-20 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPLP1 antibody 21636-1-AP | Download protocol |
| IHC protocol for RPLP1 antibody 21636-1-AP | Download protocol |
| IF protocol for RPLP1 antibody 21636-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Virulence RPLP1, an NS4B-interacting protein, enhances production of CSFV through promoting translation of viral genome.
| ||
Mol Hum Reprod 60S acidic ribosomal protein P1 (RPLP1) is elevated in human endometriotic tissue and in a murine model of endometriosis and is essential for endometriotic epithelial cell survival in vitro. | ||
Int J Mol Sci RPLP1 Is Up-Regulated in Human Adenomyosis and Endometrial Adenocarcinoma Epithelial Cells and Is Essential for Cell Survival and Migration In Vitro | ||
Am J Cancer Res Unveiling the prognostic implications of RPLP1 upregulation in osteosarcoma | ||
Cell Rep Ribosome customization and functional diversification among P-stalk proteins regulate late poxvirus protein synthesis |
