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Ribophorin I Polyclonal antibody
Ribophorin I Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, K-562 cells, HeLa cells |
| Positive IHC detected in | mouse lung tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12894-1-AP targets Ribophorin I in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, yeast |
| Immunogen | Ribophorin I fusion protein Ag3576 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ribophorin I |
| Synonyms | RPN1, RPN-I, Ribophorin-1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit |
| Calculated Molecular Weight | 69 kDa |
| Observed Molecular Weight | 66-69 kDa |
| GenBank Accession Number | BC010839 |
| Gene Symbol | RPN1 |
| Gene ID (NCBI) | 6184 |
| RRID | AB_2170474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04843 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Ribophorin I antibody 12894-1-AP | Download protocol |
| IHC protocol for Ribophorin I antibody 12894-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis | ||
Sci Adv The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress. | ||
Nat Chem Biol Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells. | ||
Int J Mol Sci Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats. | ||
Proteomics Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action. |
