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RPP25 Polyclonal antibody

RPP25 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15461-1-AP

Print datasheet

Synonyms

RNase P protein subunit p25

验证数据展示

  • IP experiment of HeLa using 15461-1-AP

    IP experiment of HeLa using 15461-1-AP

    IP result of anti-RPP25 (IP:15461-1-AP, 4ug; Detection:15461-1-AP 1:300) with HeLa cells lysate 2800 ug.

  • WB analysis of HeLa using 15461-1-AP

    WB analysis of HeLa using 15461-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15461-1-AP (RPP25 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 15461-1-AP

    IHC staining of human cervical cancer using 15461-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human small intestine using 15461-1-AP

    IHC staining of human small intestine using 15461-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 15461-1-AP

    IF Staining of HeLa using 15461-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15461-1-AP (RPP25 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman small intestine tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15461-1-AP targets RPP25 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RPP25 fusion protein Ag7742 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribonuclease P/MRP 25kDa subunit
Synonyms RNase P protein subunit p25
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC002497
Gene Symbol RPP25
Gene ID (NCBI) 54913
RRIDAB_2878142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUL9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribonuclease P protein subunit p25(RPP25) is a component of ribonuclease P, which is a protein complex that generates mature tRNA molecular by cleaving their 5'-ends. It belongs to histone-like Alba family, also a component of RNase MRP. This subunit binds to RNA.

实验方案

Product Specific Protocols
WB protocol for RPP25 antibody 15461-1-APDownload protocol
IHC protocol for RPP25 antibody 15461-1-APDownload protocol
IF protocol for RPP25 antibody 15461-1-APDownload protocol
IP protocol for RPP25 antibody 15461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Endoribonucleolytic Cleavage of m6A-Containing RNAs by RNase P/MRP Complex.

Authors - Ok Hyun Park
View Article