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验证数据展示

  • IHC staining of human lung cancer using 18435-1-AP

    IHC staining of human lung cancer using 18435-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18435-1-AP (RPP30 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 18435-1-AP

    IHC staining of human lung cancer using 18435-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18435-1-AP (RPP30 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 18435-1-AP

    IF Staining of U-251 using 18435-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (18435-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

RPP30 Polyclonal antibody

RPP30 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 18435-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

TSG15, RNASEP2, RNaseP protein p30, RNase P subunit 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18435-1-AP targets RPP30 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPP30 fusion protein Ag8824 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribonuclease P/MRP 30kDa subunit
别名 TSG15, RNASEP2, RNaseP protein p30, RNase P subunit 2
计算分子量 268 aa, 29 kDa
GenBank蛋白编号BC006991
基因名称 RPP30
Gene ID (NCBI) 10556
RRIDAB_3669300
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78346
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPP30 is part of the polyribonuclease P complex and the ribonuclease MRP complex, and is primarily involved in the 5' end maturation of tRNA, and may also be involved in other RNA metabolic processes, including RNA synthesis, processing, and maturation. RPP30 is widely expressed in a variety of tissues, including testis, lymph nodes, etc., and is also a potential biomarker for the diagnosis and prognosis of gastric cancer.

实验方案

Product Specific Protocols
IHC protocol for RPP30 antibody 18435-1-APDownload protocol
IF protocol for RPP30 antibody 18435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols