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验证数据展示

  • IHC staining of human lung cancer using 18435-1-AP

    IHC staining of human lung cancer using 18435-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18435-1-AP (RPP30 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 18435-1-AP

    IHC staining of human lung cancer using 18435-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18435-1-AP (RPP30 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 18435-1-AP

    IF Staining of U-251 using 18435-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (18435-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

RPP30 Polyclonal antibody

RPP30 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 18435-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

TSG15, RNASEP2, RNaseP protein p30, RNase P subunit 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18435-1-AP targets RPP30 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RPP30 fusion protein Ag8824 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribonuclease P/MRP 30kDa subunit
Synonyms TSG15, RNASEP2, RNaseP protein p30, RNase P subunit 2
Calculated Molecular Weight 268 aa, 29 kDa
GenBank Accession NumberBC006991
Gene Symbol RPP30
Gene ID (NCBI) 10556
RRIDAB_3669300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78346
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPP30 is part of the polyribonuclease P complex and the ribonuclease MRP complex, and is primarily involved in the 5' end maturation of tRNA, and may also be involved in other RNA metabolic processes, including RNA synthesis, processing, and maturation. RPP30 is widely expressed in a variety of tissues, including testis, lymph nodes, etc., and is also a potential biomarker for the diagnosis and prognosis of gastric cancer.

实验方案

Product Specific Protocols
IHC protocol for RPP30 antibody 18435-1-APDownload protocol
IF protocol for RPP30 antibody 18435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols