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验证数据展示

  • WB analysis of HeLa using 24542-1-AP

    WB analysis of HeLa using 24542-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24542-1-AP (RPRD1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24542-1-AP

    IF Staining of HeLa using 24542-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24542-1-AP (RPRD1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

RPRD1B Polyclonal antibody

RPRD1B Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24542-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

C20orf77, CREPT, RPRD1B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24542-1-AP targets RPRD1B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 RPRD1B fusion protein Ag21578 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulation of nuclear pre-mRNA domain containing 1B
别名 C20orf77, CREPT, RPRD1B
计算分子量 326 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC033629
基因名称 RPRD1B
Gene ID (NCBI) 58490
RRIDAB_2879597
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQG5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPRD1B antibody 24542-1-APDownload protocol
IF protocol for RPRD1B antibody 24542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

Defects in Mitochondrial Biogenesis Drive Mitochondrial Alterations in PINK1-deficient Human Dopamine Neurons

Authors - Hu Wang
  • KO Validated
View Article