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验证数据展示

  • WB analysis using 15942-1-AP

    WB analysis using 15942-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15942-1-AP (RPS11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 15942-1-AP

    IHC staining of human stomach cancer using 15942-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15942-1-AP (RPS11 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RPS11 Polyclonal antibody

RPS11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15942-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Small ribosomal subunit protein uS17, ribosomal protein S11, 40S ribosomal protein S11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, MDA-MB-453s cells, ROS1728 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15942-1-AP targets RPS11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPS11 fusion protein Ag8346 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S11
别名 Small ribosomal subunit protein uS17, ribosomal protein S11, 40S ribosomal protein S11
计算分子量 158 aa, 18 kDa
观测分子量18 kDa
GenBank蛋白编号BC007945
基因名称 RPS11
Gene ID (NCBI) 6205
RRIDAB_3085476
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62280
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS11 is a ribosomal protein involved in ribosome biogenesis. It is a component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

实验方案

Product Specific Protocols
WB protocol for RPS11 antibody 15942-1-APDownload protocol
IHC protocol for RPS11 antibody 15942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols