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RPS11 Polyclonal antibody

RPS11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15942-1-AP

Print datasheet

Synonyms

Small ribosomal subunit protein uS17, ribosomal protein S11, 40S ribosomal protein S11

验证数据展示

  • WB analysis using 15942-1-AP

    WB analysis using 15942-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15942-1-AP (RPS11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 15942-1-AP

    IHC staining of human stomach cancer using 15942-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15942-1-AP (RPS11 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, MDA-MB-453s cells, ROS1728 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15942-1-AP targets RPS11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RPS11 fusion protein Ag8346 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S11
Synonyms Small ribosomal subunit protein uS17, ribosomal protein S11, 40S ribosomal protein S11
Calculated Molecular Weight 158 aa, 18 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC007945
Gene Symbol RPS11
Gene ID (NCBI) 6205
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62280
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS11 is a ribosomal protein involved in ribosome biogenesis. It is a component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

实验方案

Product Specific Protocols
WB protocol for RPS11 antibody 15942-1-APDownload protocol
IHC protocol for RPS11 antibody 15942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols