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RPS11 Recombinant antibody

RPS11 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240327D11

Cat no : 83517-3-RR

Print datasheet

Synonyms

Small ribosomal subunit protein uS17, ribosomal protein S11, 40S ribosomal protein S11, 240327D11

验证数据展示

  • WB analysis using 83517-3-RR

    WB analysis using 83517-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83517-3-RR (RPS11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 83517-3-RR

    IHC staining of mouse testis using 83517-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 83517-3-RR

    IHC staining of mouse testis using 83517-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 83517-3-RR

    IF Staining of HepG2 using 83517-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS11 antibody (83517-3-RR, Clone: 240327D11 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • FC experiment of MCF-7 using 83517-3-RR

    FC experiment of MCF-7 using 83517-3-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RPS11 Recombinant antibody (83517-3-RR, Clone:240327D11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83517-3-RR

    Affinity and Kinetic Characterization of 83517-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83517-3-RR against Human RPS11 were performed. The affinity constant is 5.05 nM.

经过测试的应用

Positive WB detected inROS1728 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83517-3-RR is RPS11 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RPS11 fusion protein Ag34848 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein S11
Synonyms Small ribosomal subunit protein uS17, ribosomal protein S11, 40S ribosomal protein S11, 240327D11
Calculated Molecular Weight 158 aa, 18 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC007945
Gene Symbol RPS11
Gene ID (NCBI) 6205
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP62280
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS11 is a ribosomal protein involved in ribosome biogenesis. It is a component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

实验方案

Product Specific Protocols
WB protocol for RPS11 antibody 83517-3-RRDownload protocol
IHC protocol for RPS11 antibody 83517-3-RRDownload protocol
IF protocol for RPS11 antibody 83517-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols